Abstract
The new fluorescent lipophilic moiety 11-[(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-acetyl)amino]undecanoic acid (AMCA-omegaAud-OH) was introduced by SPPS at the N-terminus of the immunodominant epitope GpMBP(74-85). FRET experiments using the new fluorescent lipopeptide demonstrate that the peptide interacts with much more affinity with the membrane compared to the lipid free analogue.
MeSH terms
-
Animals
-
Binding Sites
-
Energy Transfer
-
Fluorescent Dyes / chemical synthesis*
-
Fluorescent Dyes / chemistry*
-
Immunodominant Epitopes / chemistry
-
Immunodominant Epitopes / immunology
-
Lipid Bilayers / chemistry
-
Lipoproteins / chemistry*
-
Lipoproteins / immunology
-
Lipoproteins / metabolism
-
Membrane Lipids / chemistry
-
Membrane Lipids / metabolism
-
Membranes / chemistry*
-
Models, Molecular
-
Palmitic Acid / chemistry
-
Palmitic Acid / metabolism
-
Rats
-
Spectrometry, Fluorescence
-
Spectrometry, Mass, Electrospray Ionization
Substances
-
Fluorescent Dyes
-
Immunodominant Epitopes
-
Lipid Bilayers
-
Lipoproteins
-
Membrane Lipids
-
Palmitic Acid