Abstract
The activated type 1 insulin-like growth factor (IGF-IR) increases the expression of Id1 proteins in mouse embryo fibroblasts (MEF). Up-regulation depends on a functional receptor and on multiple pathways originating from different domains of the receptor. In MEF, Id1 protein expression is also up-regulated by serum and certain oncogenes. Signaling through Stat3 plays an important, but not exclusive, role in the up-regulation of Id1 protein levels. In all instances, the increase in Id1 protein expression is paralleled by a corresponding increase in Id1 promoter activity, as measured with a reporter gene.
(c) 2002 Elsevier Science (USA).
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Binding Sites
-
Cell Line
-
DNA-Binding Proteins / genetics*
-
DNA-Binding Proteins / metabolism
-
Fibroblasts / cytology
-
Fibroblasts / metabolism
-
Helix-Loop-Helix Motifs*
-
Inhibitor of Differentiation Protein 1
-
Insulin-Like Growth Factor I / metabolism
-
Insulin-Like Growth Factor I / pharmacology
-
Mice
-
Mitogen-Activated Protein Kinase 1 / metabolism
-
Mitogen-Activated Protein Kinase 3
-
Mitogen-Activated Protein Kinases / metabolism
-
Promoter Regions, Genetic
-
Protein Serine-Threonine Kinases*
-
Protein Structure, Tertiary
-
Proto-Oncogene Proteins / metabolism
-
Proto-Oncogene Proteins c-akt
-
Receptor, IGF Type 1 / genetics
-
Receptor, IGF Type 1 / metabolism*
-
Repressor Proteins*
-
Ribosomal Protein S6 Kinases / metabolism
-
STAT3 Transcription Factor
-
Serum Albumin, Bovine / pharmacology
-
Signal Transduction*
-
Trans-Activators / metabolism
-
Transcription Factors / genetics*
-
Up-Regulation*
Substances
-
DNA-Binding Proteins
-
Idb1 protein, mouse
-
Inhibitor of Differentiation Protein 1
-
Proto-Oncogene Proteins
-
Repressor Proteins
-
STAT3 Transcription Factor
-
Stat3 protein, mouse
-
Trans-Activators
-
Transcription Factors
-
Serum Albumin, Bovine
-
Insulin-Like Growth Factor I
-
Receptor, IGF Type 1
-
Protein Serine-Threonine Kinases
-
Proto-Oncogene Proteins c-akt
-
Ribosomal Protein S6 Kinases
-
Mitogen-Activated Protein Kinase 1
-
Mitogen-Activated Protein Kinase 3
-
Mitogen-Activated Protein Kinases