Abstract
Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2mu-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 x HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacteria / enzymology
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Biotechnology / methods
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Enzymes / genetics*
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Enzymes / isolation & purification
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Fungi / enzymology*
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Fungi / genetics
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Hot Temperature
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Kluyveromyces / enzymology
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Models, Molecular
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Molecular Sequence Data
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Polymerase Chain Reaction / methods
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Protein Conformation
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Protein Sorting Signals
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Trichoderma / enzymology
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Xylan Endo-1,3-beta-Xylosidase
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Xylosidases / biosynthesis
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Xylosidases / genetics
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Xylosidases / isolation & purification
Substances
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Enzymes
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Protein Sorting Signals
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Recombinant Proteins
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Xylosidases
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Xylan Endo-1,3-beta-Xylosidase