Isolation and characterization of cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits in Nitrosomonas sp. strain ENI-11

Biosci Biotechnol Biochem. 2002 Mar;66(3):632-5. doi: 10.1271/bbb.66.632.

Abstract

The cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large and small subunits in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11 were cloned and sequenced. The deduced gene products, CbbL and CbbS, had 93 and 87% identity with Thiobacillus intermedius CbbL and Nitrobacter winogradskyi CbbS, respectively. Expression of cbbL and cbbS in Escherichia coli led to the detection of RubisCO activity in the presence of 0.1 mM isopropyl-beta-D-thiogalactopyranoside (IPTG). To our knowledge, this is the first paper to report the genes involved in the carbon fixation reaction in chemolithotrophic ammonia-oxidizing bacteria.

MeSH terms

  • Ammonia / metabolism
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Culture Media
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Escherichia coli / genetics
  • Histone-Lysine N-Methyltransferase / genetics*
  • Molecular Sequence Data
  • Nitrosomonas / enzymology*
  • Oxidation-Reduction
  • Restriction Mapping
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Culture Media
  • DNA, Bacterial
  • Ammonia
  • ribulose-1,5-bisphosphate carboxylase-oxygenase large subunit epsilonN-methyltransferase
  • Histone-Lysine N-Methyltransferase

Associated data

  • GENBANK/AF129925