The core structure of the lipopolysaccharide from the causative agent of plague, Yersinia pestis

Evgeny V Vinogradov; Buko Lindner; Nina A Kocharova; Sof'ya N Senchenkova; Aleksander S Shashkov; Yuriy A Knirel; Otto Holst; Tat'yana A Gremyakova; Rima Z Shaikhutdinova; Andrei P Anisimov

doi:10.1016/s0008-6215(02)00074-5

The core structure of the lipopolysaccharide from the causative agent of plague, Yersinia pestis

Carbohydr Res. 2002 Apr 30;337(9):775-7. doi: 10.1016/s0008-6215(02)00074-5.

Authors

Evgeny V Vinogradov¹, Buko Lindner, Nina A Kocharova, Sof'ya N Senchenkova, Aleksander S Shashkov, Yuriy A Knirel, Otto Holst, Tat'yana A Gremyakova, Rima Z Shaikhutdinova, Andrei P Anisimov

Affiliation

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47,Moscow 119991, Russia.

PMID: 11996830
DOI: 10.1016/s0008-6215(02)00074-5

Abstract

The rough-type lipopolysaccharide (LPS) of the plague pathogen, Yersinia pestis, was studied after mild-acid and strong-alkaline degradations by chemical analyses, NMR spectroscopy and electrospray-ionization mass spectrometry, and the following structure of the core region was determined:where L-alpha-D-Hep stands for L-glycero-alpha-D-manno-heptose, Sug1 for either 3-deoxy-alpha-D-manno-oct-2-ulosonic acid (alpha-Kdo) or D-glycero-alpha-D-talo-oct-2-ulosonic acid (alpha-Ko), and Sug2 for either beta-D-galactose or D-glycero-alpha-D-manno-heptose. A minority of the LPS molecules lacks GlcNAc.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
Oligosaccharides / chemistry
Plague / microbiology
Spectrometry, Mass, Electrospray Ionization
Yersinia pestis / chemistry*

Substances

Lipopolysaccharides
Oligosaccharides