Abstract
Osmotic pressure and hydrostatic pressure can be used effectively to probe the behavior of biologically important macromolecules and their complexes. Using the two techniques requires a theoretical framework as well as knowledge of the more common pitfalls. Both are discussed in this review in the context of several examples.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Animals
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Cytochrome P-450 Enzyme System / chemistry*
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Electron Transport Complex IV / chemistry*
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Humans
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Hydrostatic Pressure
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Macromolecular Substances
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Nucleic Acids / chemistry
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Osmotic Pressure
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Phosphopyruvate Hydratase / chemistry*
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Protein Conformation
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Proteins / chemistry*
Substances
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Macromolecular Substances
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Nucleic Acids
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Proteins
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Cytochrome P-450 Enzyme System
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Electron Transport Complex IV
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Phosphopyruvate Hydratase