The effects of osmotic and hydrostatic pressures on macromolecular systems

Biochim Biophys Acta. 2002 Mar 25;1595(1-2):30-47. doi: 10.1016/s0167-4838(01)00333-8.

Abstract

Osmotic pressure and hydrostatic pressure can be used effectively to probe the behavior of biologically important macromolecules and their complexes. Using the two techniques requires a theoretical framework as well as knowledge of the more common pitfalls. Both are discussed in this review in the context of several examples.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cytochrome P-450 Enzyme System / chemistry*
  • Electron Transport Complex IV / chemistry*
  • Humans
  • Hydrostatic Pressure
  • Macromolecular Substances
  • Nucleic Acids / chemistry
  • Osmotic Pressure
  • Phosphopyruvate Hydratase / chemistry*
  • Protein Conformation
  • Proteins / chemistry*

Substances

  • Macromolecular Substances
  • Nucleic Acids
  • Proteins
  • Cytochrome P-450 Enzyme System
  • Electron Transport Complex IV
  • Phosphopyruvate Hydratase