A miniature fiber optic surface plasmon resonance sensor for fast detection of Staphylococcal enterotoxin B

Biosens Bioelectron. 2002 Jun;17(6-7):591-5. doi: 10.1016/s0956-5663(02)00013-1.

Abstract

A fiber optic surface plasmon resonance (SPR) biosensor for detection of Staphylococcal enterotoxin B (SEB) is reported. The sensor is based on spectral interrogation of surface plasmons in a miniature sensing element based on a side-polished single-mode optical fiber with a thin metal overlayer. For specific detection of SEB, the SPR sensor is functionalized with a covalently crosslinked double-layer of antibodies against SEB. The SPR biosensor is demonstrated to be able to detect ng/ml concentrations of SEB in less than 10 min.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enterotoxins / analysis*
  • Equipment Design
  • Fiber Optic Technology / instrumentation*
  • Fiber Optic Technology / methods
  • Immunoassay / instrumentation
  • Immunoassay / methods
  • Miniaturization
  • Optical Fibers
  • Sensitivity and Specificity
  • Surface Plasmon Resonance / instrumentation*
  • Surface Plasmon Resonance / methods*
  • Time Factors

Substances

  • Enterotoxins
  • enterotoxin B, staphylococcal