Retrospective detection of exposure to organophosphorus anti-cholinesterases: mass spectrometric analysis of phosphylated human butyrylcholinesterase

A Fidder; A G Hulst; D Noort; R de Ruiter; M J van der Schans; H P Benschop; J P Langenberg

doi:10.1021/tx0101806

Retrospective detection of exposure to organophosphorus anti-cholinesterases: mass spectrometric analysis of phosphylated human butyrylcholinesterase

Chem Res Toxicol. 2002 Apr;15(4):582-90. doi: 10.1021/tx0101806.

Authors

A Fidder¹, A G Hulst, D Noort, R de Ruiter, M J van der Schans, H P Benschop, J P Langenberg

Affiliation

¹ Division Chemical & Biological Protection, TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA Rijswijk, The Netherlands.

PMID: 11952345
DOI: 10.1021/tx0101806

Abstract

In this paper a novel and general procedure is presented for detection of organophosphate-inhibited human butyrylcholinesterase (HuBuChE), which is based on electrospray tandem mass spectrometric analysis of phosphylated nonapeptides obtained after pepsin digestion of the enzyme. The utility of this method is exemplified by the positive analysis of serum samples from Japanese victims of the terrorist attack with sarin in the Tokyo subway in 1995.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Butyrylcholinesterase / blood*
Cholinesterase Inhibitors / adverse effects*
Environmental Exposure / analysis*
Environmental Monitoring / methods*
Humans
Insecticides / adverse effects*
Peptide Fragments / blood
Sarin / blood
Spectrometry, Mass, Electrospray Ionization
Terrorism

Substances

Cholinesterase Inhibitors
Insecticides
Peptide Fragments
Sarin
Butyrylcholinesterase