The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a potent stimulant of mast cells

Eur J Immunol. 2002 Mar;32(3):671-6. doi: 10.1002/1521-4141(200203)32:3<671::aid-immu671>3.3.co;2-x.

Abstract

Infection by Helicobacter pylori causes an acute inflammatory response followed by a chronic infection of the human gastric mucosa. A neutrophil-activating protein (HP-NAP) has been identified in H.pylori, and its role in infection and immune response is currently under investigation. Here, we show that HP-NAP induces beta-hexosaminidase release and interleukin-6 production in peritoneal mast cells, two actions which are completely inhibited by pertussis toxin. We also show that in polarized epithelial cell monolayers HP-NAP translocates from the apical to the basolateral domain, where mast cells are located. These findings characterize HP-NAP as an inflammatory factor of H.pylori that is effective from the beginning of the inflammatory cascade.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / pathology
  • Animals
  • Bacterial Proteins / pharmacology
  • Bacterial Proteins / physiology*
  • Calcimycin / pharmacology
  • Calcium / physiology
  • Cell Polarity
  • Chemotactic Factors / pharmacology
  • Chemotactic Factors / physiology*
  • Colonic Neoplasms / pathology
  • Cytoplasmic Granules / metabolism
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Exocytosis / drug effects
  • Helicobacter pylori / physiology*
  • Histamine Release / drug effects
  • Inflammation
  • Interleukin-8 / biosynthesis
  • Ionophores / pharmacology
  • Male
  • Mast Cells / drug effects*
  • Mast Cells / enzymology
  • Mast Cells / physiology
  • Peritoneal Cavity / cytology
  • Pertussis Toxin
  • Protein Transport
  • Rats
  • Rats, Wistar
  • Tumor Cells, Cultured
  • Virulence Factors, Bordetella / pharmacology
  • beta-N-Acetylhexosaminidases / metabolism

Substances

  • Bacterial Proteins
  • Chemotactic Factors
  • Interleukin-8
  • Ionophores
  • Virulence Factors, Bordetella
  • neutrophil-activating protein A, Helicobacter pylori
  • Calcimycin
  • Pertussis Toxin
  • beta-N-Acetylhexosaminidases
  • Calcium