A recombinant carboxy-terminal domain of the protective antigen of Bacillus anthracis protects mice against anthrax infection

Helen C Flick-Smith; Nicola J Walker; Paula Gibson; Helen Bullifent; Sarah Hayward; Julie Miller; Richard W Titball; E Diane Williamson

doi:10.1128/IAI.70.3.1653-1656.2002

A recombinant carboxy-terminal domain of the protective antigen of Bacillus anthracis protects mice against anthrax infection

Infect Immun. 2002 Mar;70(3):1653-6. doi: 10.1128/IAI.70.3.1653-1656.2002.

Authors

Helen C Flick-Smith¹, Nicola J Walker, Paula Gibson, Helen Bullifent, Sarah Hayward, Julie Miller, Richard W Titball, E Diane Williamson

Affiliation

¹ Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom.

Abstract

The immunogenicity and protective efficacy of overlapping regions of the protective antigen (PA) polypeptide, cloned and expressed as glutathione S-transferase fusion proteins, have been assessed. Results show that protection can be attributed to individual domains and imply that it is domain 4 which contains the dominant protective epitopes of PA.

MeSH terms

Animals
Anthrax / mortality
Anthrax / prevention & control*
Anthrax Vaccines / immunology*
Antigens, Bacterial*
Bacterial Toxins / immunology*
Female
Mice
Peptide Fragments / immunology
Protein Structure, Tertiary
Vaccination*
Vaccines, Synthetic / immunology

Substances

Anthrax Vaccines
Antigens, Bacterial
Bacterial Toxins
Peptide Fragments
Vaccines, Synthetic
anthrax toxin