Antioxidant effects of 1,5-anhydro-D-fructose, a new natural sugar, in vitro

Planta Med. 2002 Jan;68(1):16-9. doi: 10.1055/s-2002-20050.

Abstract

The antioxidant effects of 1,5-anhydro-D-fructose (1,5-AF), a unique anhydrohexulose, were studied in 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution, in human cells along with lipid peroxidation of low-density lipoprotein (LDL). We have confirmed that 1,5-AF scavenges DPPH radicals directly in solution and inhibits the formation of hydrogen peroxide and superoxide anion, typical reactive oxygen species (ROS), induced by phorbol myristate acetate (PMA) in a dose-dependent manner in THP-1 cells. We also observed the dose-dependent antioxidant effects of 1,5-AF on copper-mediated LDL oxidation. These findings suggest that 1,5-AF might play a role in reducing the risk of atherosclerosis and may help prevent coronary heart disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Bepridil / administration & dosage
  • Bepridil / analogs & derivatives*
  • Bepridil / metabolism
  • Biological Factors
  • Biphenyl Compounds
  • Carbohydrates / pharmacology*
  • Cell Line
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / isolation & purification
  • Free Radical Scavengers / pharmacology*
  • Free Radicals / administration & dosage
  • Free Radicals / metabolism
  • Fructose / analogs & derivatives*
  • Fructose / chemistry
  • Fructose / isolation & purification
  • Fructose / pharmacology*
  • Humans
  • Hydrogen Peroxide / metabolism
  • Hydroxyl Radical / metabolism
  • Lipid Peroxidation / drug effects
  • Lipoproteins, LDL / drug effects
  • Lipoproteins, LDL / metabolism
  • Macrophages
  • Molecular Structure
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Picrates*
  • Reactive Oxygen Species / metabolism
  • Superoxides / metabolism
  • Tetradecanoylphorbol Acetate / administration & dosage

Substances

  • Antioxidants
  • Biological Factors
  • Biphenyl Compounds
  • Carbohydrates
  • Free Radical Scavengers
  • Free Radicals
  • Lipoproteins, LDL
  • Picrates
  • Reactive Oxygen Species
  • Superoxides
  • Fructose
  • Hydroxyl Radical
  • 1,5-anhydrofructose
  • Bepridil
  • Hydrogen Peroxide
  • 1,1-diphenyl-2-picrylhydrazyl
  • Tetradecanoylphorbol Acetate