Abstract
The antioxidant effects of 1,5-anhydro-D-fructose (1,5-AF), a unique anhydrohexulose, were studied in 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution, in human cells along with lipid peroxidation of low-density lipoprotein (LDL). We have confirmed that 1,5-AF scavenges DPPH radicals directly in solution and inhibits the formation of hydrogen peroxide and superoxide anion, typical reactive oxygen species (ROS), induced by phorbol myristate acetate (PMA) in a dose-dependent manner in THP-1 cells. We also observed the dose-dependent antioxidant effects of 1,5-AF on copper-mediated LDL oxidation. These findings suggest that 1,5-AF might play a role in reducing the risk of atherosclerosis and may help prevent coronary heart disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Bepridil / administration & dosage
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Bepridil / analogs & derivatives*
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Bepridil / metabolism
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Biological Factors
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Biphenyl Compounds
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Carbohydrates / pharmacology*
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Cell Line
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Cells, Cultured
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Dose-Response Relationship, Drug
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Enzyme-Linked Immunosorbent Assay
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology*
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Free Radicals / administration & dosage
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Free Radicals / metabolism
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Fructose / analogs & derivatives*
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Fructose / chemistry
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Fructose / isolation & purification
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Fructose / pharmacology*
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Humans
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Hydrogen Peroxide / metabolism
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Hydroxyl Radical / metabolism
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Lipid Peroxidation / drug effects
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Lipoproteins, LDL / drug effects
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Lipoproteins, LDL / metabolism
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Macrophages
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Molecular Structure
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Monocytes / cytology
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Monocytes / drug effects
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Monocytes / metabolism
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Picrates*
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Reactive Oxygen Species / metabolism
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Superoxides / metabolism
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Tetradecanoylphorbol Acetate / administration & dosage
Substances
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Antioxidants
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Biological Factors
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Biphenyl Compounds
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Carbohydrates
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Free Radical Scavengers
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Free Radicals
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Lipoproteins, LDL
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Picrates
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Reactive Oxygen Species
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Superoxides
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Fructose
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Hydroxyl Radical
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1,5-anhydrofructose
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Bepridil
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Hydrogen Peroxide
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1,1-diphenyl-2-picrylhydrazyl
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Tetradecanoylphorbol Acetate