Detection and analysis of antigen-specific CD8+ T cells

Immunol Res. 2001;24(3):325-32. doi: 10.1385/IR:24:3:325.

Abstract

Based on recent advances in techniques that can detect and enumerate antigen-specific CD8+ T cells, it is evident that these cells can differentially regulate CD8+ T cell effector mechanisms at the single-cell level. Interplay between effector mechanisms that are employed by antigen-specific CD8+ T cells during the immune response in vivo can be addressed with different techniques that "count" cells either directly (T cell receptor (TCR) expression) or indirectly (antigen-specific cytokine production).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Antigens / metabolism
  • CD8-Positive T-Lymphocytes / immunology*
  • Cytokines / biosynthesis
  • Cytotoxicity, Immunologic
  • Humans
  • Immunologic Techniques
  • In Vitro Techniques
  • Interferon-gamma / biosynthesis
  • Leukocyte Count
  • Mice
  • Receptors, Antigen, T-Cell / metabolism
  • Tumor Necrosis Factor-alpha / biosynthesis

Substances

  • Antigens
  • Cytokines
  • Receptors, Antigen, T-Cell
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma