Abstract
[structure: see text] We describe the synthesis of a chain-terminating mRNA cap dinucleotide and its use in the in vitro transcription of homogeneously capped RNA. Computer modeling strongly indicates that RNA capped with the new compound will be a substrate for cap-dependent translation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Dinucleoside Phosphates / chemical synthesis*
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Dinucleoside Phosphates / metabolism
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Dinucleoside Phosphates / pharmacology
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Luciferases / genetics
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Methyltransferases / metabolism
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Models, Molecular
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Protein Biosynthesis / drug effects
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RNA Cap Analogs / chemical synthesis*
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RNA Cap Analogs / metabolism
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RNA Cap Analogs / pharmacology
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RNA, Messenger / chemistry*
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RNA, Messenger / metabolism
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Transcription, Genetic / drug effects*
Substances
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Dinucleoside Phosphates
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RNA Cap Analogs
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RNA, Messenger
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Luciferases
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Methyltransferases