Synthesis and application of a chain-terminating dinucleotide mRNA cap analog

Org Lett. 2002 Jan 24;4(2):161-4. doi: 10.1021/ol0167715.

Abstract

[structure: see text] We describe the synthesis of a chain-terminating mRNA cap dinucleotide and its use in the in vitro transcription of homogeneously capped RNA. Computer modeling strongly indicates that RNA capped with the new compound will be a substrate for cap-dependent translation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Dinucleoside Phosphates / chemical synthesis*
  • Dinucleoside Phosphates / metabolism
  • Dinucleoside Phosphates / pharmacology
  • Luciferases / genetics
  • Methyltransferases / metabolism
  • Models, Molecular
  • Protein Biosynthesis / drug effects
  • RNA Cap Analogs / chemical synthesis*
  • RNA Cap Analogs / metabolism
  • RNA Cap Analogs / pharmacology
  • RNA, Messenger / chemistry*
  • RNA, Messenger / metabolism
  • Transcription, Genetic / drug effects*

Substances

  • Dinucleoside Phosphates
  • RNA Cap Analogs
  • RNA, Messenger
  • Luciferases
  • Methyltransferases