Abstract
Pseudomonas sp. strain KC (= ATCC 55595 = DSM 7136) is a denitrifying aquifer isolate that produces and secretes pyridine-2,6-bis(thiocarboxylate) (PDTC), a chelating agent that fortuitously transforms carbon tetrachloride without producing chloroform. Although KC has been used successfully for full-scale bioremediation of carbon tetrachloride, its taxonomy has proven difficult to resolve, as it retains properties of both Pseudomonas stutzeri and Pseudomonas putida. In the present work, a polyphasic approach was used to conclude that strain KC represents a new genomovar (genomovar 9) within the species P. stutzeri.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Biodegradation, Environmental
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Carbon Tetrachloride / metabolism*
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DNA Gyrase / genetics
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DNA, Ribosomal / genetics
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Fresh Water / microbiology
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Inositol / metabolism
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Molecular Sequence Data
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Nitrites / metabolism
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Nucleic Acid Hybridization
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Phenotype
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Phylogeny*
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Polymerase Chain Reaction
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Pseudomonas / classification*
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Pseudomonas / genetics*
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Pseudomonas / metabolism
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Pyridines / chemistry
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Pyridines / metabolism*
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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DNA, Ribosomal
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Nitrites
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Pyridines
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RNA, Ribosomal, 16S
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pyridine-2,6-bis(thiocarboxylic acid)
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Inositol
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Carbon Tetrachloride
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DNA Gyrase
Associated data
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GENBANK/AF063219
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GENBANK/AF067960
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GENBANK/AF356514