Abstract
Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adaptor Proteins, Signal Transducing
-
Cadherins / genetics
-
Carrier Proteins
-
DNA Methylation*
-
DNA Primers
-
Genetic Testing / methods
-
Glutathione S-Transferase pi
-
Glutathione Transferase / genetics
-
Humans
-
Isoenzymes / genetics
-
Kidney Neoplasms / genetics
-
Ligases / genetics
-
Male
-
MutL Protein Homolog 1
-
Neoplasm Proteins / genetics
-
Nuclear Proteins
-
O(6)-Methylguanine-DNA Methyltransferase / genetics
-
Polymerase Chain Reaction
-
Prostatic Neoplasms / genetics
-
Tumor Cells, Cultured
-
Tumor Suppressor Proteins*
-
Ubiquitin-Protein Ligases*
-
Urinary Bladder Neoplasms / genetics
-
Urogenital Neoplasms / genetics*
-
Von Hippel-Lindau Tumor Suppressor Protein
Substances
-
Adaptor Proteins, Signal Transducing
-
Cadherins
-
Carrier Proteins
-
DNA Primers
-
Isoenzymes
-
MLH1 protein, human
-
Neoplasm Proteins
-
Nuclear Proteins
-
Tumor Suppressor Proteins
-
O(6)-Methylguanine-DNA Methyltransferase
-
Ubiquitin-Protein Ligases
-
Von Hippel-Lindau Tumor Suppressor Protein
-
GSTP1 protein, human
-
Glutathione S-Transferase pi
-
Glutathione Transferase
-
MutL Protein Homolog 1
-
Ligases
-
VHL protein, human