Abstract
We identified genes based on screening of an Arabidopsis cDNA library for functional suppressors of mouse Bax-induced cell death of yeast cells. Interestingly, the cDNA encoding AtEBP, known as Arabidopsis thaliana ethylene-responsive element binding protein, was isolated numerous times in the functional screen (82% of all suppressors). Full-length AtEBP and its localization to the nucleus were essential for the suppression of Bax-induced cell death. Morphological abnormality of intracellular network that is a hallmark of Bax-induced cell death was attenuated by expression of AtEBP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis*
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Arabidopsis / genetics
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Cell Nucleus / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Library
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Genes, Plant
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Mice
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Plant Proteins*
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-bcl-2*
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Transformation, Genetic
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bcl-2-Associated X Protein
Substances
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Arabidopsis Proteins
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Bax protein, mouse
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DNA-Binding Proteins
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Plant Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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bcl-2-Associated X Protein
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ethylene-responsive element binding protein