Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis

E Fukusaki; T Oishi; H Tanaka; S Kajiyama; A Kobayashi

doi:10.1515/znc-2001-9-1022

Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis

Z Naturforsch C J Biosci. 2001 Sep-Oct;56(9-10):814-9. doi: 10.1515/znc-2001-9-1022.

Authors

E Fukusaki¹, T Oishi, H Tanaka, S Kajiyama, A Kobayashi

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan. fukusaki@bio.eng.osaka-u.ac.jp

PMID: 11724388
DOI: 10.1515/znc-2001-9-1022

Abstract

The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Gene Expression Profiling / methods*
Gene Expression Regulation* / drug effects*
HeLa Cells / drug effects
Humans
Oligonucleotide Array Sequence Analysis*
Paclitaxel / pharmacology*
Reverse Transcriptase Polymerase Chain Reaction*
Sequence Homology, Nucleic Acid

Substances

Paclitaxel