Connection between foreign DNA replication and induced expression of the restriction-modification system in Streptomyces aureofaciens

A Godány; J Farkasovská; G Bukovská; J Timko

doi:10.1007/BF02818532

Connection between foreign DNA replication and induced expression of the restriction-modification system in Streptomyces aureofaciens

Folia Microbiol (Praha). 2001;46(3):193-6. doi: 10.1007/BF02818532.

Authors

A Godány¹, J Farkasovská, G Bukovská, J Timko

Affiliation

¹ Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia.

PMID: 11702402
DOI: 10.1007/BF02818532

Abstract

Tetracycline-producing strains of Streptomyces aureofaciens expressed SauLPI restriction-modification (R-M) system, which recognized specific DNA sequence 5'-GCCGGC-3' (isoschizomer Nael). The activation of the second R-M system SauLPII (5'-GAGCTC-3', isoschizomer of XhoI), which was silent during the growth cycle, after a foreign DNA transfer into this strain was observed. This phenomenon was tentatively explained as a response of the cells against the exogenous DNA entering the cells. The involvement of a SOS-like response in induction of R-M system genes in S. aureofaciens strains has been considered.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophage mu / genetics
DNA Replication
Deoxyribonucleases, Type II Site-Specific / metabolism*
SOS Response, Genetics
Streptomyces aureofaciens / enzymology*
Streptomyces aureofaciens / genetics*

Substances

CTCGAG-specific type II deoxyribonucleases
Deoxyribonucleases, Type II Site-Specific