[Brain-specific sequence Hfb1 is part of the 3'-untranslated region of the human complexin 2 gene: new data from in vitro and in silico experiments]

L V Dergunova; N M Raevskaia; I P Vladychenskaia; A B Poltaraus; E A Klimov; I G Udina; G E Sulimova; S A Limborskaia

[Brain-specific sequence Hfb1 is part of the 3'-untranslated region of the human complexin 2 gene: new data from in vitro and in silico experiments]

Mol Biol (Mosk). 2001 Sep-Oct;35(5):778-86.

[Article in Russian]

Authors

L V Dergunova¹, N M Raevskaia, I P Vladychenskaia, A B Poltaraus, E A Klimov, I G Udina, G E Sulimova, S A Limborskaia

Affiliation

¹ dergunov@img.ras.ru

PMID: 11605529

Abstract

A genomic clone hybridizing with brain-specific sequence Hfb1 was isolated from a chromosome 5 consmid library. Hfb1 proved to correspond to a new gene exon which codes for a large 3'-untranslated region of the mRNA for synaptic protein complexin 2. Together with the 985-nt Hfb1 cDNA (EMBL Y15167) isolated previously from a cDNA library of the frontal cerebral cortex, the primary structure was established for genomic clone Ghfb sized more than 4 kb. A GenBank search revealed complete identity of the 5' end of Ghfb and the 3'-untranslated region (878-933) of the human complexin 2 mRNA. Large transcripts with the 5' end corresponding to the complexin 2 mRNA and the 3' end to Ghfb were detected in total mRNA of the human brain by means of RT-PCR. The size of the 3'-untranslated region of the human complexin 2 mRNA was estimated at 4 kb.

Publication types

English Abstract

MeSH terms

3' Untranslated Regions*
Adaptor Proteins, Vesicular Transport
Base Sequence
Brain / metabolism*
Chromosome Mapping
Chromosomes, Human, Pair 5
DNA, Complementary
Humans
Molecular Sequence Data
Nerve Tissue Proteins / genetics*
Reverse Transcriptase Polymerase Chain Reaction

Substances

3' Untranslated Regions
Adaptor Proteins, Vesicular Transport
DNA, Complementary
Nerve Tissue Proteins
complexin II