Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5)

J Neurosci Res. 2001 Oct 1;66(1):16-22. doi: 10.1002/jnr.1193.

Abstract

We screened for genes whose expression is significantly up- or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor-5 (FGF-5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF-5 in axotomized nerve. Axon-Schwann cell interactions decreased the steady-state level of FGF-5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF-5, which is a secreted, neuronotrophic member of the FGF family.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Axons / physiology*
  • Axotomy
  • Cell Communication / physiology
  • Cells, Cultured
  • Colforsin / pharmacology
  • Cyclic AMP / metabolism
  • Fibroblast Growth Factor 5
  • Fibroblast Growth Factors / genetics*
  • Gene Expression / drug effects
  • Gene Expression / physiology
  • Nerve Regeneration / physiology*
  • RNA, Messenger / analysis
  • Rats
  • Rats, Inbred Lew
  • Schwann Cells / cytology
  • Schwann Cells / physiology*
  • Sciatic Nerve / cytology
  • Sciatic Nerve / physiology*
  • Wallerian Degeneration / physiopathology

Substances

  • Fgf5 protein, rat
  • RNA, Messenger
  • Fibroblast Growth Factor 5
  • Colforsin
  • Fibroblast Growth Factors
  • Cyclic AMP