To screen the best kinds and doses of enhancers for Ruyi Patcher, the effect of azonc(A) and propanediol (P) on the permeation of berberine(BR) from Ruyi Patcher in vitro was studied using improved Franz diffuse cells and the permeated berberine was determined by HPLC. The result showed that 3% of A in combination with 10% of P had synergistic effect, which helped enhance the skin permeation of BR significantly(P < 0.01). Therefore, a combination of 3% A and 10% P is the best enhancer for Ruyi Patcher and the penetration process of BR could be described by one-order kinetics.