Direct MALDI-TOF MS analysis of oligonucleotides on solid support through a photolabile linker

A Meyer; N Spinelli; J C Brés; C Dell'Aquila; F Morvan; I Lefebvre; B Rayner; J L Imbach; J J Vasseur

doi:10.1081/NCN-100002469

Direct MALDI-TOF MS analysis of oligonucleotides on solid support through a photolabile linker

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):963-6. doi: 10.1081/NCN-100002469.

Authors

A Meyer¹, N Spinelli, J C Brés, C Dell'Aquila, F Morvan, I Lefebvre, B Rayner, J L Imbach, J J Vasseur

Affiliation

¹ Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM2, Université Montpellier II, Place E. Bataillon, 34095, Montpellier, France.

PMID: 11563155
DOI: 10.1081/NCN-100002469

Abstract

MALDI-TOF mass spectrometry was used to analyze oligonucleotides still anchored to long-chain alkylamine controlled-pore glass (LCAA-CPG) through a photolabile linker. This technique is useful to follow supported chemical reactions in real time and monitor by-products formation.

MeSH terms

Cross-Linking Reagents / chemistry*
Glass / chemistry
Oligonucleotides, Antisense / analysis*
Oligonucleotides, Antisense / chemical synthesis
Oligonucleotides, Antisense / chemistry
Photochemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Cross-Linking Reagents
Oligonucleotides, Antisense