Pro-oligonucleotide synthesis using allyl and allyloxycarbonyl protections: direct MALDI-TOF MS analysis on solid support

N Spinelli; J J Vasseur; Y Hayakawa; J L Imbach

doi:10.1081/NCN-100002465

Pro-oligonucleotide synthesis using allyl and allyloxycarbonyl protections: direct MALDI-TOF MS analysis on solid support

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):947-50. doi: 10.1081/NCN-100002465.

Authors

N Spinelli¹, J J Vasseur, Y Hayakawa, J L Imbach

Affiliation

¹ Lab. de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM2, Université Montpellier II, Place E. Bataillon, 34095, Montpellier, France.

PMID: 11563151
DOI: 10.1081/NCN-100002465

Abstract

The solid-support synthesis of pro-oligonucleotide heteropolymer chimeras had been performed with allyloxycarbonyl group (AOC) for the protection of nucleobases and of allyl and S-acetyl-2-thioethyl (MeSATE) for phosphate protections to respectively generate phosphodiester and MeSATE phosphotriester linkages.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Oligonucleotides / chemical synthesis*
Oligonucleotides / chemistry
Organophosphates / chemical synthesis
Organophosphates / chemistry
Organophosphorus Compounds / chemistry
Prodrugs / chemical synthesis*
Prodrugs / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Oligonucleotides
Organophosphates
Organophosphorus Compounds
Prodrugs
phosphoramidite