Interaction of oligonucleotides with cellular proteins

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):859-62. doi: 10.1081/NCN-100002446.

Abstract

Oligonucleotides (ODNs) conjugated to 4-[(N-2-chloroethyl-N-methyl)amino] benzylamine were used to investigate ODN-binding proteins in cells of different origin. The data obtained demonstrate that 68, 46, 38 and 28 kDa ODN-binding proteins are universal for tested cell lines.

MeSH terms

  • 3T3 Cells
  • Animals
  • Autoradiography
  • COS Cells
  • Cytosol / metabolism
  • HeLa Cells
  • Humans
  • K562 Cells
  • Membrane Proteins / metabolism*
  • Mice
  • Mustard Compounds / metabolism*
  • Nuclear Proteins / metabolism*
  • Oligonucleotides / metabolism*
  • Phosphorus Radioisotopes
  • Protein Binding

Substances

  • Membrane Proteins
  • Mustard Compounds
  • Nuclear Proteins
  • Oligonucleotides
  • Phosphorus Radioisotopes
  • 4(N)-(2-chloroethyl-N-methylamino)benzylamine