Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope

AIDS Res Hum Retroviruses. 2001 Sep 1;17(13):1279-83. doi: 10.1089/088922201750461348.

Abstract

One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Epitopes / chemistry
  • Epitopes / immunology*
  • Gene Products, gag / chemistry
  • Gene Products, gag / immunology*
  • HIV Antibodies / biosynthesis
  • HIV Antibodies / genetics
  • HIV Antibodies / immunology*
  • HIV Antigens / chemistry
  • HIV Antigens / immunology*
  • HIV-1 / immunology
  • HIV-2 / immunology*
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • gag Gene Products, Human Immunodeficiency Virus

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Gene Products, gag
  • HIV Antibodies
  • HIV Antigens
  • gag Gene Products, Human Immunodeficiency Virus
  • gag protein p26, Human immunodeficiency virus