Functional cross-talk of HIV-1 Tat with p53 through its C-terminal domain

Biochem Biophys Res Commun. 2001 Sep 21;287(2):556-61. doi: 10.1006/bbrc.2001.5626.

Abstract

Human immunodeficiency virus type 1 (HIV-1) Tat repressed the p53-dependent gene expression through its C-terminal domain of Tat (amino acid residues 73-86) independent of the involvement of NF-kappaB and coactivator CBP/p300. Although Tat did not directly bind to p53, this repression required the N-terminal domain of p53. In contrast, Tat and p53 cooperated in the activation of HIV-1 gene expression. Thus, the cross-talk between Tat and p53 may be linked with cellular transformation by HIV-1 infection or activation of HIV-1 replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Gene Expression / drug effects
  • Gene Expression / physiology
  • Gene Products, tat / chemistry
  • Gene Products, tat / pharmacology*
  • Gene Silencing
  • HIV Long Terminal Repeat / physiology
  • HIV-1 / chemistry*
  • HIV-1 / physiology
  • NF-kappa B / metabolism
  • Protein Structure, Tertiary
  • Transcriptional Activation / drug effects
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*
  • Virus Replication
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, tat
  • NF-kappa B
  • Tumor Suppressor Protein p53
  • tat Gene Products, Human Immunodeficiency Virus