Abstract
The viability of using thiazole orange as an alternative to ethidium bromide in a fluorescent intercalator displacement (FID) assay is explored by profiling the DNA binding affinity and sequence selectivity of netropsin. Utilizing a library of hairpin deoxyoligonucleotides containing all possible four base-pair sequences, the method provides a high resolution profile of the DNA binding properties of small molecules in a high throughput format.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-Bacterial Agents / metabolism
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Benzothiazoles
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Binding, Competitive
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DNA / metabolism*
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Ethidium / chemistry
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Ethidium / standards
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / standards*
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Gene Library
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Intercalating Agents / chemistry*
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Intercalating Agents / standards
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Ligands
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Microchemistry
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Netropsin / metabolism
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Oligodeoxyribonucleotides / metabolism
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Quinolines
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Spectrometry, Fluorescence
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Thiazoles / chemistry*
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Thiazoles / standards
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Titrimetry
Substances
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Anti-Bacterial Agents
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Benzothiazoles
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Fluorescent Dyes
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Intercalating Agents
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Ligands
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Oligodeoxyribonucleotides
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Quinolines
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Thiazoles
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thiazole orange
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Netropsin
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DNA
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Ethidium