Abstract
We used PCR to detect hepatitis C virus (HCV) RNA among supernatants of ground Culex quinquefasciatus mosquitoes that (i) had been fed HCV-positive blood, (ii) had been intrathoracically inoculated with HCV-positive blood, or (iii) were from homes of hepatitis C patients. HCV RNA was detectable under all three conditions, but it did not replicate in mosquitoes and was not detectably transmitted during feeding.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Culex / physiology
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Culex / virology*
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Feeding Behavior
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Female
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Hepacivirus / genetics
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Hepacivirus / isolation & purification*
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Hepatitis C / blood
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Hepatitis C / transmission*
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Hepatitis C / virology
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction / methods
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RNA, Viral / analysis
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RNA, Viral / blood*
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Sequence Analysis, DNA