Production of aromatic minimal polyketides by the daunorubicin polyketide synthase genes reveals the incompatibility of the heterologous DpsY and JadI cyclases

J Nat Prod. 2001 Aug;64(8):1077-80. doi: 10.1021/np010067f.

Abstract

Our investigations into whether the biosynthesis of a linearly fused ring system of an aromatic polyketide (jadomycin) could be modified to produce an angularly fused system (daunorubicin) and vice versa showed that introduction of the respective cyclases did not have the desired effect. Genes from the daunorubicin pathway produced a novel 21-carbon polyketide.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyl Coenzyme A / metabolism
  • Alcohol Oxidoreductases / metabolism
  • Anthraquinones / chemistry
  • Anti-Bacterial Agents / biosynthesis*
  • Anti-Bacterial Agents / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Chromatography, High Pressure Liquid
  • Cyclization
  • Daunorubicin / chemistry
  • Doxorubicin / chemistry
  • Gene Expression
  • Genes, Bacterial
  • Intramolecular Transferases / metabolism
  • Isoquinolines / chemistry*
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Molecular Structure
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / metabolism*
  • Plasmids
  • Pyrones / chemistry
  • Pyrones / metabolism*
  • Streptomyces / enzymology*
  • Streptomyces / genetics*
  • Streptomyces / physiology

Substances

  • Anthraquinones
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DpsY protein, Streptomyces peucetius
  • Isoquinolines
  • Multienzyme Complexes
  • Pyrones
  • SEK43
  • daunorubicin-doxorubicin polyketide synthase
  • jadomycin B
  • rabelomycin
  • Acetyl Coenzyme A
  • Doxorubicin
  • aklanonic acid
  • Alcohol Oxidoreductases
  • polyketide synthase ketoreductase
  • Intramolecular Transferases
  • jadI protein, Streptomyces
  • Daunorubicin