Determination of 4,4'-dinitrocarbanilide (DNC), the active component of the antifertility agent nicarbazin, in chicken, duck, and goose plasma

T M Primus; D J Kohler; M A Goodall; C Yoder; D Griffin; L Miller; J J Johnston

doi:10.1021/jf0102463

Determination of 4,4'-dinitrocarbanilide (DNC), the active component of the antifertility agent nicarbazin, in chicken, duck, and goose plasma

J Agric Food Chem. 2001 Aug;49(8):3589-93. doi: 10.1021/jf0102463.

Authors

T M Primus¹, D J Kohler, M A Goodall, C Yoder, D Griffin, L Miller, J J Johnston

Affiliation

¹ Analytical Chemistry Project, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, Denver, Colorado 80521, USA. thomas.m.primus@usda.gov

PMID: 11513633
DOI: 10.1021/jf0102463

Abstract

4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, and goose plasma and isolated by reversed-phase high-performance liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantified by comparison to a calibration standard. Recovery data were determined by analyzing DNC-fortified control plasma. The mean recovery of DNC in fortified chicken plasma samples was 99.7 +/- 1.9% for 0.18 and 9.1 ppm DNC, and in fortified duck and goose plasma samples was 99.5 +/- 4.9% and 101.4 +/- 4.5%, respectively, for 0.18, 9.1, and 18 ppm DNC.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Calibration
Chickens
Chromatography, High Pressure Liquid / methods
Ducks
Fertility / drug effects*
Geese
Nicarbazin / blood*
Nicarbazin / chemistry
Spectrophotometry, Ultraviolet / methods

Substances

Nicarbazin