Abstract
In the course of screening for inhibitors of intracellular trafficking of glycoprotein, a new inhibitor, F13459 was isolated from the culture broth of a Penicillium sp. It was purified using solvent extraction, silica gel, Sephadex LH-20 and ODS column chromatography. From structural analysis, F13459 was a derivative of mycophenolic acid, an inhibitor of inosine 5'-monophosphate dehydrogenase. F13459 inhibited hemagglutinin synthesis of NDV at concentrations more than 25 microg/ml. However, syncytium formation as a result of cell surface expression of F-glycoprotein of NDV was inhibited at concentrations of F13459 lower than those required for appreciable inhibition of glycoprotein synthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / isolation & purification*
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Antiviral Agents / pharmacology
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Cells, Cultured
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Cricetinae
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Enzyme Inhibitors / isolation & purification
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Enzyme Inhibitors / pharmacology
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Giant Cells / drug effects
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Hemagglutinins / biosynthesis
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Kidney / cytology
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Microscopy, Electron, Scanning
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Mycophenolic Acid / analogs & derivatives*
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Mycophenolic Acid / isolation & purification*
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Mycophenolic Acid / pharmacology*
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Newcastle disease virus / drug effects
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Newcastle disease virus / metabolism
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Penicillium / chemistry*
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Penicillium / classification
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Viral Fusion Proteins / antagonists & inhibitors
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Viral Fusion Proteins / biosynthesis
Substances
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Antiviral Agents
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Enzyme Inhibitors
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F13459
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Hemagglutinins
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Viral Fusion Proteins
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Mycophenolic Acid