Objective: To express HCV RNA polymerase and study its soluble condition and antigenicity.
Methods: We constructed expression vectors pQE-5B-Fl and pQE-5B-C21 and expressed them in E.coli (M15). We analyzed their solubility in different conditions and purified soluble pQE-5B-C21 protein by Ni-NTA column, then detected its antigenicity by ELISA and western blot.
Results: We obtained the purified soluble pQE-5B-C21 protein in the induction conditions of 18 degrees C and the protein was proved to be of good antigenicity by ELISA and western blot.
Conclusions: The RNA polymerase of HCV expressed in E.coli has good solubility and antigenicity.