DNA monolayer on gold substrates characterized by nanoparticle labeling and scanning force microscopy

Nucleic Acids Res. 2001 Aug 15;29(16):E81. doi: 10.1093/nar/29.16.e81.

Abstract

Monolayers of single-stranded DNA on gold substrates were studied by scanning force microscopy. Complementary DNA probes labeled by gold nanoparticles were applied for contrast enhancement. Substrate regions modified with DNA could be visualized in a highly specific manner. The influence of the solution concentration on the surface density of adsorbed nanoparticles could be visualized. Because individual label particles can be easily detected, this labeling technique opens the way for characterization of DNA monolayers with a lateral resolution in the nanometer range.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Probes / chemistry
  • DNA Probes / genetics
  • DNA Probes / metabolism
  • DNA Probes / ultrastructure
  • DNA, Single-Stranded / chemistry
  • DNA, Single-Stranded / genetics
  • DNA, Single-Stranded / metabolism*
  • DNA, Single-Stranded / ultrastructure*
  • Gold / analysis
  • Gold / metabolism*
  • Gold Colloid / analysis
  • Gold Colloid / metabolism*
  • Microscopy, Atomic Force*
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / genetics
  • Oligodeoxyribonucleotides / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Solutions

Substances

  • DNA Probes
  • DNA, Single-Stranded
  • Gold Colloid
  • Oligodeoxyribonucleotides
  • Solutions
  • Gold