Objective: To study the effects of 17997 on the HSV-1 DNA and protein synthesis.
Methods: DNA synthesis was determined by isopycnic separation of cellular and viral DNA in CsCl gradients centrifugation. Protein synthesis was determined by SDS-PAGE and Western blot.
Results: The inhibition rates of 0.5 micromol/L, 1.0 micromol/L, 2.0 micromol/L and 4.0 micromol/L of 17997 on HSV-1 DNA synthesis were 63.1%,74. 1%, 93.9% and 100%, respectively. 4.0 micromol/L of 17997 did not inhibit VERO cell DNA synthesis. The above concentrations of 17997 did not show any inhibitory effects on cellular protein synthesis, but selectively inhibited on viral protein synthesis.
Conclusions: 17997 selectively displayed inhibitive effects on HSV-1 DNA and protein synthesis.