Abstract
We have analyzed the blood B cell subpopulations of children with systemic lupus erythematosus (SLE) and healthy controls. We found that the normal recirculating mature B cell pool is composed of four subsets: conventional naive and memory B cells, a novel B cell subset with pregerminal center phenotype (IgD(+)CD38(+)centerin(+)), and a plasma cell precursor subset (CD20(-)CD19(+/low)CD27(+/++) CD38(++)). In SLE patients, naive and memory B cells (CD20(+)CD38(-)) are approximately 90% reduced, whereas oligoclonal plasma cell precursors are 3-fold expanded, independently of disease activity and modality of therapy. Pregerminal center cells in SLE are decreased to a lesser extent than conventional B cells, and therefore represent the predominant blood B cell subset in a number of patients. Thus, SLE is associated with major blood B cell subset alterations.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ADP-ribosyl Cyclase
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ADP-ribosyl Cyclase 1
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Adolescent
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Adult
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Antigens, CD*
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Antigens, CD19 / biosynthesis
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Antigens, CD20 / biosynthesis
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Antigens, Differentiation / biosynthesis
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B-Lymphocyte Subsets / cytology
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B-Lymphocyte Subsets / pathology*
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Cell Differentiation / immunology
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Cell Survival / immunology
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Child
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Clone Cells
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Female
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Germinal Center / immunology
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Germinal Center / pathology*
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Hematopoietic Stem Cells / immunology
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Hematopoietic Stem Cells / pathology*
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Humans
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Immunologic Memory
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Immunophenotyping
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Interphase / immunology
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Lupus Erythematosus, Systemic / blood*
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Lupus Erythematosus, Systemic / immunology*
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Lupus Erythematosus, Systemic / pathology
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Lymphocyte Count
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Lymphocytosis / immunology
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Lymphocytosis / pathology*
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Lymphopenia / blood
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Lymphopenia / pathology
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Male
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Membrane Glycoproteins
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NAD+ Nucleosidase / biosynthesis
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Plasma Cells / pathology*
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T-Lymphocyte Subsets / pathology
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / biosynthesis
Substances
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Antigens, CD
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Antigens, CD19
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Antigens, CD20
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Antigens, Differentiation
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Membrane Glycoproteins
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Tumor Necrosis Factor Receptor Superfamily, Member 7
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ADP-ribosyl Cyclase
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CD38 protein, human
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NAD+ Nucleosidase
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ADP-ribosyl Cyclase 1