A technique of heterotopic, infrarenal heart transplantation with double anastomosis in mice

T Doenst; C Schlensak; J L Kobba; F Beyersdorf

doi:10.1016/s1053-2498(00)00248-5

A technique of heterotopic, infrarenal heart transplantation with double anastomosis in mice

J Heart Lung Transplant. 2001 Jul;20(7):762-5. doi: 10.1016/s1053-2498(00)00248-5.

Authors

T Doenst¹, C Schlensak, J L Kobba, F Beyersdorf

Affiliation

¹ Department of Cardiovascular Surgery, University of Freiburg, Freiburg, Germany. doenst@ch11.ukl.uni-freiburg.de

PMID: 11448806
DOI: 10.1016/s1053-2498(00)00248-5

Abstract

Mouse heart transplantation is frequently used to investigate mechanisms of rejection and immune response. A fully vascularized technique is presented in which the graft ascending aorta is anastomosed end-to-side to the recipient's aorta and the pulmonary aorta is anastomosed end-to-side to the inferior vena cava using 11-0 Ethilon suture. We suggest that this double anastomosis technique is a valuable tool to investigate immune mechanisms in mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anastomosis, Surgical / methods*
Animals
Aorta, Abdominal / surgery
Graft Survival
Heart Arrest, Induced / adverse effects
Heart Transplantation / adverse effects
Heart Transplantation / methods*
Mice
Mice, Inbred BALB C
Models, Animal
Myocardial Ischemia / etiology
Myocardial Ischemia / pathology
Myocardium / pathology
Transplantation, Heterotopic / methods*
Vena Cava, Inferior / surgery*