Evidence for the presence of DNA-binding proteins involved in regulation of the gene expression of indole-3-pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum lipoferum FS

K Yagi; T Chujo; H Nojiri; T Omori; M Nishiyama; H Yamane

doi:10.1271/bbb.65.1265

Evidence for the presence of DNA-binding proteins involved in regulation of the gene expression of indole-3-pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum lipoferum FS

Biosci Biotechnol Biochem. 2001 May;65(5):1265-9. doi: 10.1271/bbb.65.1265.

Authors

K Yagi¹, T Chujo, H Nojiri, T Omori, M Nishiyama, H Yamane

Affiliation

¹ Biotechnology Research Center, The University of Tokyo, Japan.

PMID: 11440156
DOI: 10.1271/bbb.65.1265

Abstract

We isolated the ipdc gene coding for indole-3-pyruvic acid decarboxylase (IPDC), a key enzyme in the indole-3-pyruvic acid pathway for indole-3-acetic acid biosynthesis, in the plant growth-promoting rhizobacterium Azospirillum lipoferum FS. Gel mobility-shift assay showed the presence of two DNA-binding proteins that might be involved in regulation of the ipdc gene expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azospirillum / enzymology
Azospirillum / genetics
Azospirillum / metabolism*
Base Sequence
Carboxy-Lyases / genetics*
DNA, Bacterial
DNA-Binding Proteins / metabolism
DNA-Binding Proteins / physiology*
Gene Expression Regulation, Bacterial / physiology*
Gene Expression Regulation, Enzymologic / physiology*
Indoleacetic Acids / metabolism*
Molecular Sequence Data

Substances

DNA, Bacterial
DNA-Binding Proteins
Indoleacetic Acids
indoleacetic acid
indolepyruvate decarboxylase
Carboxy-Lyases