Human urotensin II-induced contraction and arterial smooth muscle cell proliferation are mediated by RhoA and Rho-kinase

Circ Res. 2001 Jun 8;88(11):1102-4. doi: 10.1161/hh1101.092034.

Abstract

The aim of this work was to investigate the coupling of human urotensin II (hU-II) to RhoA activation and regulation of RhoA-dependent functions. The use of the Rho-kinase inhibitor Y-27632 and the development of a membrane-permeant RhoA inhibitor (TAT-C3) allowed us to demonstrate that hU-II induced arterial smooth muscle contraction, actin stress fiber formation, and proliferation through the activation of the small GTPase RhoA and its downstream effector Rho-kinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP Ribose Transferases / genetics
  • ADP Ribose Transferases / metabolism
  • ADP Ribose Transferases / pharmacology
  • Amides / pharmacology
  • Animals
  • Aorta, Thoracic / cytology
  • Aorta, Thoracic / metabolism
  • Botulinum Toxins*
  • Cell Division / drug effects
  • Cells, Cultured
  • Cytoskeleton / drug effects
  • Cytoskeleton / metabolism
  • Dose-Response Relationship, Drug
  • Enzyme Activation / physiology
  • Enzyme Inhibitors / pharmacology
  • Humans
  • In Vitro Techniques
  • Intracellular Signaling Peptides and Proteins
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / metabolism*
  • Norepinephrine / antagonists & inhibitors
  • Norepinephrine / pharmacology
  • Potassium Chloride / pharmacology
  • Protein Serine-Threonine Kinases / antagonists & inhibitors
  • Protein Serine-Threonine Kinases / metabolism*
  • Pyridines / pharmacology
  • Rats
  • Rats, Wistar
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Fusion Proteins / pharmacology
  • Signal Transduction / drug effects
  • Signal Transduction / physiology
  • Urotensins / metabolism
  • Urotensins / pharmacology*
  • Vasoconstriction / drug effects
  • Vasoconstriction / physiology*
  • Vasoconstrictor Agents / antagonists & inhibitors
  • Vasoconstrictor Agents / pharmacology
  • rho-Associated Kinases
  • rhoA GTP-Binding Protein / antagonists & inhibitors
  • rhoA GTP-Binding Protein / metabolism*

Substances

  • Amides
  • Enzyme Inhibitors
  • Intracellular Signaling Peptides and Proteins
  • Pyridines
  • Recombinant Fusion Proteins
  • Urotensins
  • Vasoconstrictor Agents
  • Y 27632
  • Potassium Chloride
  • urotensin II
  • ADP Ribose Transferases
  • exoenzyme C3, Clostridium botulinum
  • Protein Serine-Threonine Kinases
  • rho-Associated Kinases
  • Botulinum Toxins
  • rhoA GTP-Binding Protein
  • Norepinephrine