Synthesis and properties of oligonucleotides containing 8-bromo-2'-deoxyguanosine

Nucleosides Nucleotides Nucleic Acids. 2001 Mar;20(3):251-60. doi: 10.1081/ncn-100002085.

Abstract

The preparation of oligonucleotides containing 8-bromo-2'-deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2'-deoxyguanosine paired with natural bases were much less stable.

MeSH terms

  • Chromatography / methods
  • Circular Dichroism
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / analysis
  • Deoxyguanosine / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Nucleic Acid Conformation
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Organophosphorus Compounds / chemistry

Substances

  • 8-bromo-2'-deoxyguanosine
  • Oligonucleotides
  • Organophosphorus Compounds
  • phosphoramidite
  • Deoxyguanosine