Abstract
The preparation of oligonucleotides containing 8-bromo-2'-deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2'-deoxyguanosine paired with natural bases were much less stable.
MeSH terms
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Chromatography / methods
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Circular Dichroism
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Deoxyguanosine / analogs & derivatives
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Deoxyguanosine / analysis
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Deoxyguanosine / chemistry*
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Nucleic Acid Conformation
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Oligonucleotides / chemical synthesis*
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Oligonucleotides / chemistry
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Organophosphorus Compounds / chemistry
Substances
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8-bromo-2'-deoxyguanosine
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Oligonucleotides
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Organophosphorus Compounds
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phosphoramidite
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Deoxyguanosine