Abstract
In this work we present evidence for a novel diffusible extracellular factor that modulates gene expression in Bacillus subtilis. The factor was found when studying the regulation of the fatR-cyp102A3 operon. In a Spo0A(-) mutant expression of the fatR-cyp102A3 operon was almost abolished. The fatR-cyp102A3 expression defect of a Spo0A(-) mutant could be overcome either by a mutation in the abrB gene or by a diffusible substance excreted by wild-type, abrB mutant and abrB-spo0A double mutant strains.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / genetics*
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Bacterial Proteins*
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Biological Transport
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Cytochrome P-450 Enzyme System / genetics
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DNA-Binding Proteins / genetics*
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Diffusion
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Gene Deletion
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Gene Expression Regulation, Bacterial*
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Gene Silencing
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Genetic Complementation Test
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Mixed Function Oxygenases / genetics
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Mutation
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NADPH-Ferrihemoprotein Reductase
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Oligopeptides / metabolism
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Operon
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Promoter Regions, Genetic / genetics*
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Transcription Factors / genetics*
Substances
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AbrB protein, Bacillus subtilis
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Bacterial Proteins
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DNA-Binding Proteins
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Oligopeptides
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Transcription Factors
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Cytochrome P-450 Enzyme System
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Mixed Function Oxygenases
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NADPH-Ferrihemoprotein Reductase
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flavocytochrome P450 BM3 monoxygenases