A novel diffusible substance can overcome the apparent AbrB repression of the Bacillus subtilis fatR promoter

FEMS Microbiol Lett. 2001 May 30;199(2):197-202. doi: 10.1111/j.1574-6968.2001.tb10674.x.

Abstract

In this work we present evidence for a novel diffusible extracellular factor that modulates gene expression in Bacillus subtilis. The factor was found when studying the regulation of the fatR-cyp102A3 operon. In a Spo0A(-) mutant expression of the fatR-cyp102A3 operon was almost abolished. The fatR-cyp102A3 expression defect of a Spo0A(-) mutant could be overcome either by a mutation in the abrB gene or by a diffusible substance excreted by wild-type, abrB mutant and abrB-spo0A double mutant strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics*
  • Bacterial Proteins*
  • Biological Transport
  • Cytochrome P-450 Enzyme System / genetics
  • DNA-Binding Proteins / genetics*
  • Diffusion
  • Gene Deletion
  • Gene Expression Regulation, Bacterial*
  • Gene Silencing
  • Genetic Complementation Test
  • Mixed Function Oxygenases / genetics
  • Mutation
  • NADPH-Ferrihemoprotein Reductase
  • Oligopeptides / metabolism
  • Operon
  • Promoter Regions, Genetic / genetics*
  • Transcription Factors / genetics*

Substances

  • AbrB protein, Bacillus subtilis
  • Bacterial Proteins
  • DNA-Binding Proteins
  • Oligopeptides
  • Transcription Factors
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • NADPH-Ferrihemoprotein Reductase
  • flavocytochrome P450 BM3 monoxygenases