Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

J A Wagenaar; M A van Bergen; D G Newell; R Grogono-Thomas; B Duim

doi:10.1128/JCM.39.6.2283-2286.2001

Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

J Clin Microbiol. 2001 Jun;39(6):2283-6. doi: 10.1128/JCM.39.6.2283-2286.2001.

Authors

J A Wagenaar¹, M A van Bergen, D G Newell, R Grogono-Thomas, B Duim

Affiliation

¹ Institute for Animal Science and Health, 8200 AB Lelystad, The Netherlands. j.a.wagenaar@id.wag-ur.nl

Abstract

A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Typing Techniques
Campylobacter fetus / classification*
Campylobacter fetus / genetics
Campylobacter fetus / metabolism
DNA Fingerprinting / methods*
Genotype
Phenotype
Polymerase Chain Reaction / methods*
Polymorphism, Genetic