Production of human papillomavirus type 16 early proteins in Bac-To-Bac Expression System (GibcoBRL)

M Schmidt; A K Olejnik; K Stepień; A M Olejnik; W Butowska; W Grajek; J B Warchoł; A Gozdzicka-Józefiak

Production of human papillomavirus type 16 early proteins in Bac-To-Bac Expression System (GibcoBRL)

Folia Histochem Cytobiol. 2001;39(2):125-6.

Authors

M Schmidt¹, A K Olejnik, K Stepień, A M Olejnik, W Butowska, W Grajek, J B Warchoł, A Gozdzicka-Józefiak

Affiliation

¹ Department of Molecular Virology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland. mschmidt@amu.edu.pl

PMID: 11374788

Abstract

Human papillomavirus type 16 (HPV16) is a major agent in cervical cancer etiology. Its early proteins are responsible for virus persistence, replication and initiation of neoplastic disease. In the present study we describe a use of baculovirus-insect cell expression system for production and study of HPV16 E2 and E4 proteins. The E2 protein binds specifically to viral DNA and E4 protein shows characteristic cytopathic effects on cells.

MeSH terms

Animals
Autoradiography
Baculoviridae / genetics*
Cell Line
Cell Nucleus / genetics
DNA-Binding Proteins*
Electrophoresis, Polyacrylamide Gel
Humans
Insecta
Oncogene Proteins, Viral / biosynthesis*
Oncogene Proteins, Viral / genetics
Papillomaviridae / metabolism*
Plasmids / genetics

Substances

DNA-Binding Proteins
E2 protein, Human papillomavirus type 16
Oncogene Proteins, Viral