Abstract
The presence of the multidrug efflux pump SmeDEF was assessed in a collection of clinical isolates of Stenotrophomonas maltophilia. All isolates encoded this pump, as demonstrated by PCR. Forty-seven percent of the strains overproduced a protein of the same size that was immunoreactive against an anti-SmeF antibody, and 33% overexpressed the gene semD when they were tested by reverse transcription-PCR. A correlation between smeDEF overexpression and antibiotic resistance was observed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins*
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Carrier Proteins / genetics*
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Drug Resistance, Multiple / genetics
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Gene Expression Regulation, Bacterial*
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Membrane Transport Proteins*
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Microbial Sensitivity Tests
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Stenotrophomonas maltophilia / drug effects
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Stenotrophomonas maltophilia / genetics*
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Stenotrophomonas maltophilia / isolation & purification
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carrier Proteins
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Membrane Transport Proteins
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SmeD protein, Stenotrophomonas maltophilia
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SmeE protein, Stenotrophomonas maltophilia
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SmeF protein, Stenotrophomonas maltophilia