Comparison of sequence analysis and the INNO-LiPA HBV DR line probe assay for detection of lamivudine-resistant hepatitis B virus strains in patients under various clinical conditions

S W Aberle; J Kletzmayr; B Watschinger; B Schmied; N Vetter; E Puchhammer-Stöckl

doi:10.1128/JCM.39.5.1972-1974.2001

Comparison of sequence analysis and the INNO-LiPA HBV DR line probe assay for detection of lamivudine-resistant hepatitis B virus strains in patients under various clinical conditions

J Clin Microbiol. 2001 May;39(5):1972-4. doi: 10.1128/JCM.39.5.1972-1974.2001.

Authors

S W Aberle¹, J Kletzmayr, B Watschinger, B Schmied, N Vetter, E Puchhammer-Stöckl

Affiliation

¹ Institute of Virology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria. stephan.aberle@univie.ac.at

Abstract

A line probe assay (INNO-LiPA HBV DR) detecting drug-resistant hepatitis B virus (HBV) strains was evaluated. Results concordant with sequence analysis were obtained with 48 of 56 serum samples from HBV-infected patients undergoing lamivudine therapy. In eight cases, additional minor subpopulations could be identified by the line probe assay.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Antiviral Agents / pharmacology*
Antiviral Agents / therapeutic use
DNA, Viral / analysis
DNA, Viral / genetics
DNA-Directed DNA Polymerase / genetics*
Drug Resistance, Microbial / genetics
Hepatitis B virus / drug effects*
Hepatitis B virus / enzymology
Hepatitis B virus / genetics
Hepatitis B, Chronic / drug therapy
Hepatitis B, Chronic / virology
Humans
Kidney Transplantation / adverse effects
Lamivudine / pharmacology*
Lamivudine / therapeutic use
Mutation
Sequence Analysis, DNA*

Substances

Antiviral Agents
DNA, Viral
Lamivudine
DNA-Directed DNA Polymerase