Human immunodeficiency virus type 1-specific immunity after genetic immunization is enhanced by modification of Gag and Pol expression

Y Huang; W P Kong; G J Nabel

doi:10.1128/JVI.75.10.4947-4951.2001

Human immunodeficiency virus type 1-specific immunity after genetic immunization is enhanced by modification of Gag and Pol expression

J Virol. 2001 May;75(10):4947-51. doi: 10.1128/JVI.75.10.4947-4951.2001.

Authors

Y Huang¹, W P Kong, G J Nabel

Affiliation

¹ Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

Abstract

Immunity to human immunodeficiency virus virion-like structures or a polyprotein has been examined after DNA immunization with Rev-independent expression vectors. A Gag-Pol fusion protein stimulated cytotoxic T lymphocyte and antibody responses to Gag and Pol, while a Gag-Pol pseudoparticle did not elicit substantial Pol responses. This fusion protein may be useful for AIDS vaccines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AIDS Vaccines / immunology*
Animals
Artificial Gene Fusion
Cell Line, Transformed
Female
Genes, gag / immunology*
Genes, pol / immunology*
HIV Antibodies / blood
HIV Core Protein p24 / immunology
HIV-1 / immunology*
Humans
Mice
Mice, Inbred BALB C
Vaccination
Vaccines, DNA / immunology*

Substances

AIDS Vaccines
HIV Antibodies
HIV Core Protein p24
Vaccines, DNA