The objective of this N balance study was to determine the potential for improving the efficiency and rate of dietary N utilization in Holstein steers by feeding an amino acid-balanced mixture of animal by-product protein sources in combination with urea. The Beef NRC 1996 Model Level 2 was used to formulate a corn-based (86:14 concentrate-hay) control diet with soybean meal as the primary N supplement that would provide ME and metabolizable protein (MP) allowable ADG of 1.4 kg in 250-kg steers with an estrogenic implant and fed an ionophore. A combination of porcine meat and bone meal, fish meal, hydrolyzed feather meal, and blood meal was also formulated as an undegradable intake protein (UIP) blend to complement those amino acids (AA) derived from microbial protein synthesis. Four steers with an average initial BW of 259 kg were assigned in a 4 x 4 Latin square design to treatments consisting of control, two levels of UIP inclusion (2.6 and 5.2%; DM basis) in combination with urea, and a negative control "urea diet" containing no UIP and no SBM. The steers were fed at hourly intervals 95% of ad libitum intake and were injected with 500 microg of estradiol-17beta twice daily. Nitrogen intakes were 155, 160, 162, and 145 g/d, and N balances were 47, 51, 42, and 47 g/d when the 0, 2.6, 5.2% UIP and the urea diets were fed, respectively. Nitrogen balance was reduced with the 5.2% UIP diet (P < 0.05), and was less than the capacity estimate derived from abosmasal casein infusion studies. Apparent N digestibilities averaged 69%, but DM, OM, and nonstructural carbohydrate digestibilities were significantly reduced for the urea diet. Feeding 5.2% UIP in the diet reduced (P < 0.05) the biological value from 46 to 38%, which was accompanied by a significant elevation of plasma urea N. Results indicate that genetic capacity for N retention was approximately 51 g/d. Results demonstrate that use of an AA-balanced blend of animal by-product protein sources did not improve the efficiency of dietary N usage when added to corn-based diets formulated with the Beef NRC 1996 Model Level 2 to meet nutrient requirements of rapidly growing steers. Using urea as the only N supplement achieved equal rate and efficiency of N use.