Abstract
Encephalitozoon spores were isolated in a primary tissue culture of the kidneys from an encephalitozoonosis-suspected rabbit in a municipal zoo in Hokkaido. The isolated spores were morphologically characteristic of microsporidial ones in chromotrope stain, and proven to be E. cuniculi by a polymerase chain reaction (PCR) with a species-specific primer set and by direct DNA sequencing of the PCR products.
MeSH terms
-
Animals
-
Base Sequence
-
Culture Techniques
-
DNA, Protozoan / chemistry
-
DNA, Protozoan / isolation & purification
-
Encephalitozoon cuniculi / chemistry
-
Encephalitozoon cuniculi / genetics
-
Encephalitozoon cuniculi / isolation & purification*
-
Encephalitozoonosis / diagnosis
-
Encephalitozoonosis / parasitology*
-
Kidney / parasitology
-
Kidney / pathology
-
Molecular Sequence Data
-
Polymerase Chain Reaction / veterinary
-
Rabbits / parasitology*
-
Sequence Alignment
-
Sequence Analysis, DNA