[Construction of peptide mimetics of an epitope of the human immunodeficiency virus (HIV-1) gp41 protein, recognized by virus-neutralizing antibodies 2F5]

Mol Biol (Mosk). 2001 Jan-Feb;35(1):146-51.
[Article in Russian]

Abstract

A phase peptide library was screened with virus-neutralizing monoclonal antibodies (MCA) 2F5 which recognize a conserved epitope of HIV-1 gp41. Phages that expose peptides specifically binding with MCA 2F5 were selected by ELISA. Amino acid sequence analysis revealed a homology to region 662-671 of HIV-1 HB10 gp160 for most peptides. The major role in recognition was ascribed to Asp-664, Lys-665, and Trp-666. The epitope-mimicking peptides were tested for immunogenicity. Antibodies to gp41 were detected in serum of immunized rabbits.

Publication types

  • English Abstract

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / blood
  • Antibodies, Monoclonal / immunology*
  • Base Sequence
  • DNA Primers
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / chemistry*
  • HIV Envelope Protein gp41 / chemistry*
  • HIV Envelope Protein gp41 / immunology
  • HIV-1 / immunology*
  • Molecular Mimicry*
  • Molecular Sequence Data
  • Neutralization Tests
  • Peptides / chemistry*
  • Rabbits
  • Sequence Homology, Amino Acid

Substances

  • Antibodies, Monoclonal
  • DNA Primers
  • Epitopes
  • HIV Envelope Protein gp41
  • Peptides