In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteins

Microbiology (Reading). 2001 Feb;147(Pt 2):507-513. doi: 10.1099/00221287-147-2-507.

Abstract

A new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M, a mild symptom line derived from OY-W. We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 7.0 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromosomes, Bacterial / genetics*
  • Chrysanthemum cinerariifolium / microbiology*
  • Cloning, Molecular
  • DNA Helicases / genetics
  • DNA Helicases / metabolism*
  • DNA Replication
  • DNA, Bacterial / genetics
  • DNA-Binding Proteins*
  • Geminiviridae / genetics*
  • Geminiviridae / metabolism
  • Molecular Sequence Data
  • Plant Diseases / microbiology
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tenericutes / genetics*
  • Tenericutes / metabolism
  • Tenericutes / pathogenicity
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*

Substances

  • DNA, Bacterial
  • DNA-Binding Proteins
  • Trans-Activators
  • replication initiator protein
  • DNA Helicases

Associated data

  • GENBANK/AB010426