[Regeneration of HNP transgenic alfalfa plants by Agrobacterium mediated gene transfer]

D Y Lü; Y L Fan; M M Yu; S X Tang; N Hou; Q Wang

[Regeneration of HNP transgenic alfalfa plants by Agrobacterium mediated gene transfer]

Yi Chuan Xue Bao. 2000;27(4):331-7.

[Article in Chinese]

Authors

D Y Lü¹, Y L Fan, M M Yu, S X Tang, N Hou, Q Wang

Affiliation

¹ Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.

PMID: 11147352

Abstract

Transgenic plants regenerated from cotyledons of M. sativa L. infected using Agrobacterium tumefaciens A281 with plasmid pBF649 containing a gene encoding protein of high sulfur-amino acid content (HNP) were obtained successfully. The plants grew and fertiled well in field. Cotyledon explants were better recipient for transformation of M. sativa L. Environment of suitable temperature (15 degrees C) and high humidity on high viability of the plants transplanted into soil were essential conditions.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids, Sulfur / genetics*
Gene Transfer Techniques
Medicago sativa / genetics*
Medicago sativa / physiology
Plants, Genetically Modified / genetics*
Plants, Genetically Modified / physiology
Regeneration
Rhizobium*

Substances

Amino Acids, Sulfur